ข้อมูลเทคโนโลยีที่ใช้ในการผลิต

ข้อมูลเทคโนโลยีที่ใช้ในการผลิต

Vasileios Pothakos a,1, Luc De Vuyst a,⁎,1, Sophia Jiyuan Zhang a, Florac De Bruyna, Marko Verce a, Julio Torres b,c, Michael Callanan d, Cyril Moccand d, Stefan Weckx a
-
Temporal shotgun metagenomics of an Ecuadorian coffee fermentation process highlights the predominance of lactic acid bacteria
-
Temporal shotgunmetagenomics ofmultiple samples of an Arabicawet coffee fermentation process, which was examined microbiologically and metabolomically before, was performed to complement these microbiological and biochemical data with an in-depth in silico analysis of the structure and functions of the coffee microbiome. The taxonomic analysis of a massive sequence dataset of ca. 16 Gbp identified >150 microbial species and distinguished three successive, microbial phases: (i) enterobacteria, acetic acid bacteria, and some yeasts prevailing at the start; (ii) lactic acid bacteria (LAB) in the second phase; and (iii) acid-tolerant LAB at the end of the fermentation process. The functional analysis of prokaryotic genes, based on ca. 138,000 coding sequences, facilitated the gene repertoire overview of this ecosystem, showcasing differences in each fermentation stage. Initial prototrophic characters, plant cell wall-degrading activities, and pectinolytic activities were followed by amore auxotrophic and saccharolytic microbial profile, due to an increasing relative abundance of LAB. The almost full genome of 22 bacterial species was reconstructed and additional coffee-specific features were identified. This temporal metagenomic analysis of a case of reproducible wet coffee fermentation processes carried out in a coffee plantation near Nanegal, Ecuador, constitutes a breakthrough for the study of wet coffee processing and the contribution of the growth and activities of different microorganisms, in particular LAB, to this process.
-
Coffee bean fermentation Wet processing Shotgun metagenomics Taxonomic analysis Functional analysis Leuconostoc pseudomesenteroides Lactobacillus
2020